Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Listeria monocytogenes is one of the most virulent foodborne pathogens, responsible for an increasing amount of deaths worldwide annually. Listeriosis is the major cause of death among foodborne bacterial pathogens.

Infections by Listeria monocytogenes can happen in any stages of food processing. Listeria monocytogenes can be found in the fecal matter, soil and waste waters. It is also found in the intestinal tract of many animals including mammals, birds, fish, insects, reptiles. As such it easily gets into the food chain as a contaminant of raw food.
Whilst it is killed by heat processes such as pasteurisation it is innately resistant to harsh environmental conditions e.g. it survives low pH, high salt and can grow at refrigeration conditions as well as producing biofilms. This can result in it persisting in food processing environments with the potential to contaminate foods. Foods that receive no further cooking or heat treatments such as ready-to-eat foods are an issue.
When Listeria is detected as part of monitoring, even if it is low levels below the legal limit, the source contamination should be investigated as L. monocytogenes can persist in food processing environments for several years. Factory hygiene in general is important, particularly cleaning of equipment - equipment should be able to be dismantled for thorough cleaning.

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytogenes and Listeria spp. is specified, and in Part 2, the enumeration of these organisms in food, animal feed and environmental samples is described. The revised Part 1 edition cancels and replaces the first edition EN ISO 11290-1:1996 and incorporates the amendment 1 from 2004. Part 2 cancels and replaces the first edition of EN ISO 11290-2:1998 and incorporates the amendment 1 from 2004. Compared to the previous edition, the main changes are considered as major. In the revised EN ISO 11290:2017, the scope in both Parts 1 and 2 has been extended to enable also the detection and enumeration of Listeria spp.

Listeria monocytogenes and Listeria spp. detection according to the EN ISO 11290-1:2017 standard
The main changes, compared to ISO 11290-1:1996, are the following:
— Scope extended to include Listeria spp. detection. The title has changed accordingly.
— Primary enrichment in half-Fraser broth: incubation for 25 h ± 1 h.
— Secondary enrichment in Fraser broth: incubation for 24 h ± 2 h.
— Half-Fraser and Fraser broths may be refrigerated before transfer or isolation on selective agar for a maximum of 72 h.
— Storage of isolation plates: incubated plates can be refrigerated for a maximum of two days before reading.
— Microscopic aspect for confirmation is optional if the isolation agar allows distinction between pathogenic and non-pathogenic Listeria spp.
— CAMP test and catalase test are optional.
— Inclusion of new performance characteristics.

Listeria monocytogenes and Listeria spp. enumeration according to the revised EN ISO 11290-2:2017 standard
The main changes, compared to ISO 11290‑2:1998, are the following:
— Enumeration of Listeria spp. is included in the scope. Title is changed accordingly.
— Primary suspension with buffered peptone water, half-Fraser broth with or without supplements, and all appropriate diluents referred to in ISO 6887 (all parts).
— Resuscitation step (incubation of initial suspension of sample 1 h ± 5 min at 20 °C ± 2 °C) deleted.
— Microscopic aspect, catalase and CAMP test for confirmation are optional.
— Inclusion of new performance characteristics.

Compliance to revised ISO 11290:2017
We have implemented all the requirements described in both part 1 and part 2 of the revised EN ISO 11290:2017.
Our dehydrated culture media, ready-to-use media in agar plates, tubes, bottles, bags are all compliant with EN ISO 11133:2014. All information regarding the reference standard compliance are indicated in the product technical sheets, available on our website.

Check our products for Listeria monocytogenes and Listeria spp.:

Code

Category

Name

Packaging

414090

Tubes - Bottles

Listeria Enrichment Broth

6 bottle x 225 ml

26131

Tubes - Bottles

LISTERIA FRASER BROTH

100 tubes

20131/9

Tubes - Bottles

LISTERIA FRASER BROTH

10 tube x 9 ml

413980

Tubes - Bottles

LISTERIA FRASER BROTH

6 bottle x 225 ml

24131

Tubes - Bottles

LISTERIA FRASER BROTH

20 tube

24471

Tubes - Bottles

Listeria Motility Medium

20 tube

412070

Tubes - Bottles

LISTERIA SELECTIVE BROTH

6 bottle x 200 mL

24435

Tubes - Bottles

LISTERIA U.V.M. BROTH

20 tube

461030

Tubes - Bottles

LISTERIA UVM 1 BROTH

6 bottle x 225 ml

461020

Tubes - Bottles

LISTERIA UVM 2 BROTH

6 bottle x 225 ml

33087

Tubes - Bottles

LISTERIA PALCAM AGAR

10 tubes

499010

Ready Bags

LISTERIA UVM MOD BROTH - BAG

3 x 3 L

499015

Ready Bags

LISTERIA UVM MOD BROTH - BAG

3 x 5 L

499020

Ready Bags

LISTERIA DEMI FRASER BROTH - BAG

3 x 3 L

499025

Ready Bags

LISTERIA DEMI FRASER BROTH - BAG

3 x 5 L

10041

90 mm agar plates

LISTERIA PALCAM AGAR

20 plate 90 mm

10225

140 mm agar plates

LISTERIA PALCAM AGAR

10 plate 140 mm

11230

90 mm agar plates

LISTERIA AESCULIN AGAR

20 plate 90 mm

11230*

90 mm agar plates

LISTERIA AESCULIN AGAR

100 plate 90 mm

10223

Chromogenic plates

O.A. LISTERIA AGAR

10 plate 140 mm

10620

Chromogenic plates

O.A. LISTERIA AGAR

20 plate 90 mm

610601

Dehydrated Culture Media

O.A. LISTERIA AGAR

500 g

620601

Dehydrated Culture Media

O.A. LISTERIA AGAR

100 g

81074

Supplements

O.A. LISTERIA Supplement

4+4 vials x 500 mL

610324

Dehydrated Culture Media

BUFFERED LISTERIA ENRICHMENT BROTH

500 g

610031

Dehydrated Culture Media

LISTERIA UVM 1 BROTH

500 g

620031

Dehydrated Culture Media

LISTERIA UVM 1 BROTH

100 g

6100315

Dehydrated Culture Media

LISTERIA UVM 1 BROTH

5 Kg

620045

Dehydrated Culture Media

LISTERIA UVM 2 BROTH

100 g

610045

Dehydrated Culture Media

LISTERIA UVM 2 BROTH

500 g

620168

Dehydrated Culture Media

LISTERIA PALCAM AGAR

100 g

610168

Dehydrated Culture Media

LISTERIA PALCAM AGAR

500 g

620167

Dehydrated Culture Media

LISTERIA OXFORD AGAR

100 g

610167

Dehydrated Culture Media

LISTERIA OXFORD AGAR

500 g

610231

Dehydrated Culture Media

LISTERIA OXFORD AGAR BASE

500 g

610166

Dehydrated Culture Media

LISTERIA FRASER BROTH

500 g

620166

Dehydrated Culture Media

LISTERIA FRASER BROTH

100 g

610618

Dehydrated Culture Media

LISTERIA FRASER BROTH II

500 g

610326

Dehydrated Culture Media

LISTERIA ENRICHMENT B.BASE (Lovett)

500 g

620326

Dehydrated Culture Media

LISTERIA ENRICHMENT B.BASE (Lovett)

100 g

81045

Supplements

LISTERIA FOR ENRICHMENT SUPPLEMENT(FDA/IDF)

10 vials

81028

Supplements

LISTERIA FRASER supplement

10 vials

81042

Supplements

LISTERIA FRASER supplement (1125mg)

10 vials

81628A

Supplements

LISTERIA FRASER supplement (FAC-NA)

5 + 5 vials

80304

Supplements

Listeria Fraser Supplement (liquid)

5 x 10 mL

81027

Supplements

LISTERIA OXFORD supplement

10 vials

81629

Supplements

LISTERIA OXFORD w CEF-FOS-COL-CYC-ACR suppl

10 vials

81026

Supplements

LISTERIA PALCAM supplement

10 vials

81044

Supplements

MODIFIED LISTERIA ENRICHMENT SUPP.

10 vials

88010

Tests for microbial ID

LISTERIA MONO TEST

20 test

96146

Latex test

LISTERIA LATEX KIT

50 tests

96319

Rapid test

LISTERIA MONOCYTOGENES FECAL/FOOD CARD

10 cards

 

Contam Swab Listeria (ref. 86102) is a swab based test with culture media for easy microbial count and identification directly from environmental surfaces. 



Benefits
✓ Easy to use
✓ Clear color changes
✓ No additional reagents
✓ Results in 18/24 hours
✓ 10 CFU/mL detection limit

Listeria System 18R (ref. 71640) is a gallery for the biochemical identification of Listeria spp. (Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria seeligeri, Listeria welshimeri, Listeria grayi sub. grayi, Listeria grayi sub. murrayi, Jonesia denitrificans ).

Benefits
✓ Complete identification
✓ Cost effective
✓ Results in 18-24 hours
✓ Clear and well defined color changes
✓ Easy interpretation, visually or by automated readers.

Liofilchem’s Food System (ref. 71680) is an innovative solution, validated to ISO 16140 for Listeria monocytogenes and Salmonella spp. detection, that can simplify the workflow of your microbiological testing laboratory: https://www.liofilchem.com/en/products/featured-products/food-system.html

We also produce the freeze-dried CultiControl™ pellets for Quality Control and Quanti-CultiControl™ for Growth Promotion Testing, Microbial Limits Testing, Microbial Enumeration Testing:

Code

Category

Name

Packaging

89101

ATCC

Listeria grayi ATCC ® 25401

5 pellets

89101

ATCC

Listeria grayi ATCC ® 25401

5 pellets

89029

ATCC

Listeria innocua ATCC ® 33090

5 pellets

89030

ATCC

Listeria ivanovii ATCC ® 19119

5 pellets

89085

ATCC

Listeria monocytogenes ATCC ® 13932

5 pellets

89188

ATCC

Listeria monocytogenes ATCC ® 15313

5 pellets

89031

ATCC

Listeria monocytogenes ATCC ® 19111

5 pellets

89051

ATCC

Listeria monocytogenes ATCC ® 19115

5 pellets

89148

ATCC

Listeria monocytogenes ATCC ® 35152

5 pellets

89060

ATCC

Listeria monocytogenes ATCC ® 7644

5 pellets

89143

ATCC

Listeria monocytogenes ATCC ® BAA-751

5 pellets

89525

ATCC

Listeria monocytogenes ATCC ® 19115

1 pellet + 1 rehydrating fluid

This website uses cookies, which may include third party cookies, to improve your browsing experience and deliver personalized marketing content.
To find out more, including how to disable the cookies, read our Cookie Policy.
By continuing to browse this website, you agree to our use of cookies.