Isolation, detection, transportation
| Description | Packaging | Ref. |
|---|---|---|
|
Helicobacter pylori Agar Selective medium for isolating Helicobacter pylori.
|
20 petri dishes
|
10082 |
|
Helicobacter pylori Test Selective broth for isolating Helicobacter pylori from bioptical and fecal specimens.
|
20 test
|
20090 |
|
Other: Columbia Agar (Sheep blood 5%) Mueller Hinton Agar II (Sheep blood 5%) Mueller Hinton Fastidious Agar (Horse blood 5% + 20 mg/L β-NAD) Brain Heart Infusion Agar Brain Heart Infusion Broth |
20 petri dishes 20 petri dishes 20 petri dishes 20 petri dishes 20 petri dishes |
11025 10131 10132 10060 24104 |
|
H.pylori CARD Rapid test for antobodies IgG to Helicobacter pylori determination in human serum. |
20 Card |
96455 |
|
FECAL H.pylori ANTIGEN CARD Rapid test for qualitative detection of Helicobacter pylori antigen in human fecal specimens. |
20 Card
|
97803 |
Antimicrobial Susceptibility Testing
Helicobacter pylori MIC Test Strip method
Medium
Mueller Hinton II Agar (Sheep blood 5%) or Mueller Hinton Fastidious Agar (Horse blood 5% + 20 mg/L β-NAD)
Inoculum
72 h (or older) viable colonies are suspended in broth (Mueller Hinton or other) supplemented with 5% serum; adjust turbidity to 3 McFarland. Use 1 MIC Test Strip per 90 mm plate; position the handle of the strip against the edge of the plate.
Incubation
35 ± 2°C/ microaerophilic (atmosphere produced by a gas-generating system suitable for Campylobacter)
72 hours (or longer i.e. until a visible inhibition ellipse is seen).
For metronidazole, a 24 hours anaerobic pre-incubation followed by 48 hours or longer microaerophilic incubation has been recommended by some investigators as a better option.
Reading precautions
H. pylori colonies are pin-point, translucent and difficult to see. Tilt the plate and/or use oblique light or a magnifying glass when reading the M.I.C. endpoint. Bactericidal drugs: interpret the M.I.C. at the point of complete inhibition of all growth, including microcolonies, hazes and isolated colonies.
Bacteriostatic drugs: interpret the M.I.C. of hazy zone edges at 80% inhibition.
| μg/mL | strips per pack | ref. |
EUCAST |
||
|---|---|---|---|---|---|
| S≤ | R> | ||||
|
MIC Test Strip AMOXICILLIN
|
0.016 - 256 |
10 |
920211 |
|
|
|
MIC Test Strip LEVOFLOXACIN |
0.002 - 32 |
10 |
920811 |
|
|
|
MIC Test Strip CLARITHROMYCIN
|
0.016 - 256 |
10 |
920481 |
|
|
|
MIC Test Strip TETRACYCLINE |
0.016 - 256 |
10 30 100 |
921141 92114 921140 |
1 |
1 |
|
MIC Test Strip METRONIDAZOLE |
0.016 - 256 |
10 30 100 |
920871 92087 920870 |
8 |
8 |
|
MIC Test Strip RIFAMPICIN |
0.016 - 256 |
10 30 100 |
920251 92025 920250 |
1 |
1 |