Chromogenic culture media for detecting antimicrobial resistance mechanisms



Chromatic Colistin

Chromogenic medium for detection of colistin-resistant organisms from clinical specimens and other materials.


Chromatic Colistin is a selective chromogenic medium used for the isolation and differentiation of Colistinresistant Gram-negative bacteria. The medium is intended for screening of faecal samples and can also be used to confirm colistin resistance of isolates from non-selective and non-chromogenic culture media.

Packaging Ref.

20 plates

11640

Chromatic Costilin






Chromatic ESBL

Chromatic ESBL

Selective chromogenic medium for screening Gram-negative ESBL-producing bacteria.

ESBL (Extended Spectrum β-Lactamases) are enzymes that confer resistance to penicillins, extended-spectrum third generation cephalosporins (C3G) and monobactams. The ESBL-producing Enterobacteriaceae are responsible of severe hospital-acquired infections. The correct and early detection of ESBL-producing microorganisms is critical for addressing to the most appropriate antimicrobial therapy and avoiding the spread of infections. Chromatic ESBL medium contains a mixture of chromogenic compounds and antibiotics that allow the the growth of ESBL-producing bacteria while inhibit the other bacteria, including the ampC-positive. While the AmpC-positive bacteria can still be treated with certain beta-lactamase-stable antibiotics, the presence of an ESBL infection seriously limits treatment options because of the wide resistance acquired.

Description Packaging Ref.

Chromatic ESBL

20 plates

11622

Chromatic ESBL+AmpC

20 plates

11629

Chromatic ESBL

500 g

610629

Chromatic ESBL

100 g

620629

Chromatic ESBL supplement

 

81089

Chromatic ESBL+AmpC supplement

 

81090






Chromatic CRE

Screening medium for the detection of Carbapenem-Resistant Enterobacteriaceae.

Chromatic CRE contains a mixture of carbapenems for screening a wide variety of carbapenem-resistance mechanisms and provides presumptive identification of E. coli and the Klebsiella, Enterobacter, Serratia and Citrobacter (KESC) group directly from clinical specimens.
Carbapenems, successfully used to treat multi-resistant Gram-negative bacterial infections, including ESBL positive strains, are not efficacious against the Enterobacteriaceae resistant to carbapenems, thus generating a significant risk of hospital-acquired infections.

Packaging Ref.

20 plates

11619

500 g

611619

100 g

621619

Chromatic CRE supplement

81088

Chromatic CRE






Chromatic OXA-48

Chromatic OXA-48

Selective chromogenic medium for the screening of OXA-48 type Carbapenem-Resistant Enterobacteriaceae.

OXA-48 CRE are multi-resistant bacteria potentially responsible of hospital infections. The detection of OXA-48 CRE carriers by Chromatic OXA-48 can prevent and help surveil those infections.
Chromatic OXA-48, with its own proprietary formulation, includes an antimicrobial agents and chromogenic substrata mixture that allows the selective growth of OXA-48 CRE and the identification of Escherichia coli (red color), Klebsiella spp. (Blue-violet), Enterobacter spp. (blue-green), Citrobacter spp. (blue with red halo).

Packaging Ref.

20 plates

11631






Chromatic VRE

Chromogenic medium for screening vancomycin-resistant enterococci.

Chromatic VRE contains a mixture of antibiotics including vancomycin for screening Vancomycin-resistant enterococci (VRE)  and provides presumptive identification of Enterococcus faecium and Enterococcus faecalis directly from clinical specimens.
VRE have recently been recognized as one of the most severe cause of nosocomial infections.
An intrinsic resistance (vanC, vanD, vanE, vanF etc) is found in E. gallinarum and E. casseliflavus/E. flavescens and shows low resistance to vancomycin. Instead, an acquired resistance of vancomycin in enterococci (vanA & vanB types) is mostly detected in E. faecium and E. faecalis.
The prompt detection of Vancomycin-resistance of E. faecium and E. faecalis is basic for avoiding the spread of this resistance to more virulent such as S. aureus

Packaging Ref.

20 plates

11621

Chromatic VRE






Chromatic MRSA

Chromatic MRSA

Selective chromogenic medium for isolating methicillin- resistant Staphylococcus aureus.

Methicillin resistant Staphylococcus aureus (MRSA) caused an increasing number of hospital infections in the recent years.
A wide range of antimicrobial compounds, including the beta-lactam antibiotics, result unsuccessful for treating the methicillin resistant S. aureus.

Packaging Ref.

20 plates

10599

500 g

610615

100 g

620615

Chromatic MRSA supplement

81078






Chromatic MH

Chromogenic Muller Hinton for presumptive identification and susceptibility testing of various microorganisms from clinical specimens.

In the Intensive Care Unit the mortality rates for VAP, sepsis, surgical site or intra-abdominal, catheter related infections are critically high. Direct M.I.C. on CSF, positive blood culture bottles and other specimens from critical patients and direct M.I.C. on bronchial aspirates from patients with VAP can contribute with timely and essential information to save the life of patients.

Packaging Ref.

20 plates

11618

500 g

611618

100 g

621618

Chromatic MH






Combo plates available

Combo plates available #

# Minimum orders are required. Custom versions on request.

Description Packaging Ref.

Chromatic Staph.aureus / Chromatic MRSA

20 plates

18007

Chromatic CRE / Chromatic OXA-48

20 plates

18023

Chromatic Detection / Chromatic ESBL

20 plates

18011

Chromatic CRE / Chromatic ESBL

20 plates

100 plates

18021

18021*






References

 

Carriage of extended-spectrum beta-lactamases and carbapenem-resistant Gram-negative bacilli in children under five suffering from complicated severe acute malnutrition hospitalised in a district hospital in Niger.
Ms. G. Lai , Dr. C. Langendorf, MD O. Guindo, Dr. S. Isanaka, Dr. PhD Nada Malou, Dr. C. Mambula, Dr. B. Hassan, Prof. Dr. T. R. Walsh
ECCMID 2019


Validation of a novel Chromatic Agar Selective against Colistin-resistant Enterobacteriaceae

E. Widlake, R. Marrollo, F. Brovarone, F. Demetrio, D. Vitagliano, F. Brocco, TR Walsh, E. Carretto, JM. Tyrrell
ASM Microbe 2019


Evaluation of Chromatic OXA-48 medium for direct detection of OXA-48 producing Enterobacteriaceae from rectal swabs

A. Antonelli, L. Henrici De Angelis, J. Tyrrell, T. Walsh, D. Vitagliano, F. Brocco, G. M. Rossolini and M. M. D'Andrea
ECCMID 2016


Evaluation of a highly selective chromogenic medium to screen for OXA-type carbapenemase-positive Enterobacteriaceae

JM Tyrrell, F. Demetrio, D. Vitagliano, F. Brocco, M. Wootton, Timothy R. Walsh.
ECCMID 2015, poster no. 151


Direct determination of antimicrobial susceptibility of Gram Negative Bacilli from respiratory samples on chromogenic agar plates with gradient antibiotic strips

C. d'Humières, M. Belhiba, F. Brocco, A. Andremont, N. Grall
ECCMID 2014, Poster no. 348


Liofilchem® Chromatic VRE and vancomycin MIC Test Strip detected glycopeptide resistance in a vanB neonatal Enterococcus faecium isolate showing alternate vancomycin susceptibility and resistance with bioMérieux Vitek2

Vincenzo Savini, Roberta Marrollo, Eleonora Coclite, Paola Fusilli, Carmine D'Incecco, Paolo Fazii, Giovanni Gherardi Int J Clin
Exp Pathol 2014;7(9):6274-6277


New broth - chromogenic Mueller Hinton agar procedure for urine samples - next-day result of Enterobacteriaceae antimicrobial susceptibility testing
Iztok Strumbelj
ECCMID 2013, poster no. 1547


A novel, direct susceptibility testing method of sonicated vascular prosthetic graft samples by combination of Liofilchem® MIC Test Strip and chromogenic agar plates

F.H. M’Zali1, L. Stecken2, X. Berard1,2, A. Quinart2 and F. Sztark1,2
ECCMID 2013


Evaluation of a new method for detecting KPC-producing bacteria based on the combination of a screening medium and a gradient diffusion system

F. Brocco, D. Vitagliano, F. Demetrio
ECCMID 2013


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